{"id":927,"date":"2020-09-21T09:55:50","date_gmt":"2020-09-21T09:55:50","guid":{"rendered":"http:\/\/www.aroundlab.org\/news\/?p=927"},"modified":"2020-09-21T10:34:21","modified_gmt":"2020-09-21T10:34:21","slug":"nota-applicativa-igiene-n-06-monitoraggio-microbiologico-delle-superfici-con-piastre-a-contatto","status":"publish","type":"post","link":"https:\/\/www.aroundlab.org\/news\/nota-applicativa-igiene-n-06-monitoraggio-microbiologico-delle-superfici-con-piastre-a-contatto\/","title":{"rendered":"Nota Applicativa Igiene N.06 \u2013 Monitoraggio microbiologico delle superfici con piastre a contatto"},"content":{"rendered":"

Nota Applicativa Igiene N.06<\/span><\/h1>\n

Monitoraggio microbiologico delle superfici con piastre a contatto<\/span><\/h1>\n
\n

L\u2019impiego delle piastre a contatto\u00a0 (Contact Plate, RODAC) risulta pi\u00f9 obiettivo e riproducibile in abbinamento al sistema \u201cRODAC-WEIGHT\u201d\u00a0<\/b>in quanto viene eliminata la soggettivit\u00e0 dell\u2019operatore. \u00a0Si riportano i suggerimenti presentati nella pubblicazione \u201cIl Monitoraggio Microbiologico negli Ambienti di Lavoro\u201d Edizione 2005 dell\u2019 INAIL (P. Anzidei, L. Frusteri, R. Giovinazzo, E. Guerrera, D. Sarto, N. Todaro, F. Venanzetti).<\/span><\/p>\n

RODAC-WEIGHT<\/span><\/h3>\n

\u201cIl campionamento delle superfici viene eseguito mediante l\u2019applicazione di piastre Petri tipo a contatto (cio\u00e8 con la superficie del terreno adatta per l\u2019adesione con la superficie da monitorare), riempite di idoneo terreno di coltura, esercitando una lieve pressione della piastra sulla superficie da monitorare. Si raccomanda l\u2019utilizzo di un applicatore temporizzato a peso standardizzato secondo le norme ISO (ad esempio il \u201cRODAC-WEIGHT\u201d, piuttosto che l\u2019effettuazione manuale della applicazione. Infatti i coefficiente di variazione sono risultati molto pi\u00f9 bassi quando si \u00e8 utilizzato il \u201cRODAC-WEIGHT\u201d piuttosto che il campionamento \u201cmanuale\u201d per il controllo delle medesime superfici. La durata standard della applicazione \u00e8 di 10 secondi. E\u2019 consigliabile effettuare i prelievi in triplo sulla medesima superficie in esame, campionando in tre punti vicini significativi per la valutazione dei livelli medi di contaminazione.\u201d<\/span><\/p>\n

\u201cTerreni e parametri microbiologici\u201d<\/span><\/h3>\n\n\n\n\n\n\n\n\n\n
\n

PARAMETRO<\/b><\/span><\/h3>\n<\/td>\n

\n

TERRENO<\/b><\/span><\/h3>\n<\/td>\n<\/tr>\n

Conta batterica totale psicrofila<\/span><\/td>\nPlate Count AgarTryptic Soy Agar<\/span><\/td>\n<\/tr>\n
Conta batterica totale mesofila<\/span><\/td>\nPlate Count AgarTryptic Soy Agar<\/span><\/td>\n<\/tr>\n
Conta fungina totale\u00a0 con inibizione della popolazione microbica contaminante<\/span><\/td>\nSabourad Agar + Cloramfenicolo<\/span><\/td>\n<\/tr>\n
Stafilococchi<\/span><\/td>\nMannitol Salt Agar<\/span><\/td>\n<\/tr>\n
Batteri Gram negativi<\/span><\/td>\nMac Conkey Agar<\/span><\/td>\n<\/tr>\n
Pseudomonas<\/i>\u00a0spp<\/span><\/td>\nCetrimide Agar<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u201cCalcolo delle Unit\u00e0 Formanti Colonie\u201d su superfici<\/span><\/h3>\n

La densit\u00e0 microbica, espressa in termini di UFC\/100 centimetri quadrati, viene calcolata dividendo il risultato del conteggio (N, numero di colonie per piastra) per l\u2019area di contatto della piastra con la superficie in esame (24 centimetri quadrati) e moltiplicando il risultato per 100.<\/span><\/p>\n

UFC\/100 centimetri quadrati = N \/ 24 x 100.\u201d<\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Nota Applicativa Igiene N.06 Monitoraggio microbiologico delle superfici con piastre a contatto L\u2019impiego delle piastre a contatto\u00a0 (Contact Plate, RODAC) risulta pi\u00f9 obiettivo e riproducibile in abbinamento al sistema \u201cRODAC-WEIGHT\u201d\u00a0in […]<\/p>\n","protected":false},"author":1,"featured_media":970,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"_price":"","_stock":"","_tribe_ticket_header":"","_tribe_default_ticket_provider":"","_tribe_ticket_capacity":"0","_ticket_start_date":"","_ticket_end_date":"","_tribe_ticket_show_description":"","_tribe_ticket_show_not_going":false,"_tribe_ticket_use_global_stock":"","_tribe_ticket_global_stock_level":"","_global_stock_mode":"","_global_stock_cap":"","_tribe_rsvp_for_event":"","_tribe_ticket_going_count":"","_tribe_ticket_not_going_count":"","_tribe_tickets_list":"[]","_tribe_ticket_has_attendee_info_fields":false,"footnotes":""},"categories":[11,10,18],"tags":[19],"class_list":["post-927","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-it","category-nota-applicativa","category-nota-applicativa-igiene","tag-nota-applicativa-igiene"],"yoast_head":"\nNota 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